Disease | laryngeal carcinoma |
Comorbidity | C0280324|epidermoid carcinoma of the larynx |
Sentences | 1 |
PubMedID- 20441585 | The hep-2 cell line, originally established from an epidermoid carcinoma of the larynx (atcc, rockville, maryland, usa), was seeded at a density of 1 × 106 cells/ml per 75 cm2 culture flask (corning, ny, usa) in medium mem-earle (cultilab, campinas, sp, brazil), ph 7.5, supplemented with 20% fetal calf serum (cultilab), 1% non-essential amino acids, 0.1% antibiotic/antimycotic (invitrogen corporation, carlsbad, ca, usa), and cultured at 37°c in a humid atmosphere of 5% co2. |
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